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@ -1,7 +1,8 @@
@@ -1,7 +1,8 @@
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Sensospot Data Parser |
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===================== |
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Parsing the numerical output from Sensovation Sensospot image analysis. |
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Parsing the numerical output from Sensovation Sensospot image analysis and some |
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other useful functions for working with the data. |
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## Example: |
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@ -10,15 +11,71 @@ Parsing the numerical output from Sensovation Sensospot image analysis.
@@ -10,15 +11,71 @@ Parsing the numerical output from Sensovation Sensospot image analysis.
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import sensospot_data |
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# read the raw data of a folder |
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raw_data = sensospot_data.process_folder(<path to results directory>) |
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raw_data = sensospot_data.parse_folder(<path to results directory>) |
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# apply an exposure map to add more data: |
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# key relates to column "Exposure.Id" |
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# values are (Exposure.Channel, Exposure.Time) |
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exposure_map = { |
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1: ("Cy3", 100), |
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2: ("Cy5", 150), |
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3: ("Cy5", 15), |
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} |
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enhanced_data = sensospot_data.apply_exposure_map(raw_data, exposure_map) |
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# split the measurement according to channels |
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channels = sensospot_data.split_channels(raw_data [, optional_exposure_map]) |
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channels = sensospot_data.split_data_frame(enhanced_data "Exposure.Channel") |
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# normalize one channel to a specific exposure time |
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cy5_normalized = sensospot_data.normalize_channel(channels["cy5"], normalized_time=25) |
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# merge the two cy5 measurements together, creating an extended dynamic range |
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cy5_xdr = sensospot_data.create_xdr(channels["cy5"], normalized_time=25) |
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``` |
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## Avaliable functions: |
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from .parser import parse_file, parse_folder # noqa: F401 |
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- **parse_folder(path_to_folder)** |
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Searches the folder for parsable .csv files, parses them into one big pandas |
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data frame and will add additional meta data from parameters folder, if |
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it is present. |
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- **parse_file(path_to_csv_file)** |
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Parses the csv file into a pandas data frame and will add additional some |
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meta data from the file name. Is internally also used by `parse_folder()` |
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- **split_data_frame(data_frame, column)** |
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Splits a data frame based on the unique values of a column. Will return a |
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dict, with the unique values as keys and the corresponding data frame as |
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value |
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- **apply_exposure_map(data_frame, exposure_map)** |
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Adds information about the channel and exposure time to a data frame, based |
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on the exposure id. Will get bonus karma points, if the named tuple |
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`ExposureInfo` is used: |
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`{1:ExposureInfo("Cy3", 100), 2:ExposureInfo("Cy3", 100), }` |
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- **ExposureInfo(exposure_channel, exposure_time)** |
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A named tuple for defining an exposure map. Usage will increase readability |
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and karma points. |
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- **blend(data_frame, [column="Spot.Mean", limit=0.5])** |
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If provided with a data frame with multiple exposure times for the same |
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exposure channel, the function will blend theese two times together based |
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on given column and limit. |
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- **normalize_values(data_frame, [normalized_time=None])** |
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Adds new columns to the data frame with intensity values recalculated to the |
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normalized exposure time. If no time is given, the max exposure time is used. |
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- **create_xdr(data_frame, [normalized_time=None, column="Spot.Mean", limit=0.5])** |
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This combines the methods `blend()` and `normalize_values()` into one call. |
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What a joy! |
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## CLI |
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For the (propably) most important function, there is even a cli command |
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```sh |
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Usage: parse_sensospot_data [OPTIONS] SOURCE |
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Arguments: |
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SOURCE: Folder with Sensospot measurement |
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Options: |
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-o, --outfile TEXT Output file name, relative to SOURCE, defaults to 'raw_data.h5' |
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--help Show this message and exit. |
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``` |
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## Development |
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