Parsing the numerical output from Sensovation SensoSpot image analysis.
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""" Column name definitions """
# original, unmodified column names
COL_NAME_POS_X = "Pos.X"
COL_NAME_POS_Y = "Pos.Y"
COL_NAME_BKG_MEAN = "Bkg.Mean"
COL_NAME_SPOT_MEAN = "Spot.Mean"
COL_NAME_BKG_MEDIAN = "Bkg.Median"
COL_NAME_SPOT_MEDIAN = "Spot.Median"
COL_NAME_BKG_STDDEV = "Bkg.StdDev"
COL_NAME_SPOT_STDDEV = "Spot.StdDev"
COL_NAME_BKG_SUM = "Bkg.Sum"
COL_NAME_SPOT_SUM = "Spot.Sum"
COL_NAME_BKG_AREA = "Bkg.Area"
COL_NAME_SPOT_AREA = "Spot.Area"
COL_NAME_SPOT_SAT = "Spot.Sat. (%)"
COL_NAME_POS_NOM_X = "Pos.Nom.X"
COL_NAME_POS_NOM_Y = "Pos.Nom.Y"
# replacement column names
COL_NAME_POS_ID = "Pos.Id"
COL_NAME_SPOT_FOUND = "Spot.Found"
COL_NAME_SPOT_DIAMETER = "Spot.Diameter"
# additional column
COL_NAME_SPOT_OVERFLOW = "Spot.Overflow"
# well information
COL_NAME_WELL_ROW = "Well.Row"
COL_NAME_WELL_COLUMN = "Well.Column"
# parsed measurement parameter information
COL_NAME_PARAMETERS_CHANNEL = "Parameters.Channel"
COL_NAME_PARAMETERS_TIME = "Parameters.Time"
# applied exposure info
COL_NAME_EXPOSURE_ID = "Exposure.Id"
COL_NAME_EXPOSURE_CHANNEL = "Exposure.Channel"
COL_NAME_EXPOSURE_TIME = "Exposure.Time"
RAW_DATA_COLUMN_SET = {
COL_NAME_POS_X,
COL_NAME_POS_Y,
COL_NAME_BKG_MEAN,
COL_NAME_SPOT_MEAN,
COL_NAME_BKG_MEDIAN,
COL_NAME_SPOT_MEDIAN,
COL_NAME_BKG_STDDEV,
COL_NAME_SPOT_STDDEV,
COL_NAME_BKG_SUM,
COL_NAME_SPOT_SUM,
COL_NAME_BKG_AREA ,
COL_NAME_SPOT_AREA,
COL_NAME_SPOT_SAT,
COL_NAME_POS_NOM_X,
COL_NAME_POS_NOM_Y,
COL_NAME_POS_ID,
COL_NAME_SPOT_FOUND,
COL_NAME_SPOT_DIAMETER,
COL_NAME_WELL_ROW,
COL_NAME_WELL_COLUMN,
COL_NAME_PARAMETERS_CHANNEL,
COL_NAME_PARAMETERS_TIME,
COL_NAME_EXPOSURE_ID
}
# normalized columns
COL_NAME_NORMALIZED_EXPOSURE_TIME = f"Normalized.{COL_NAME_EXPOSURE_TIME}"
COL_NAME_NORMALIZED_BKG_MEAN = f"Normalized.{COL_NAME_BKG_MEAN}"
COL_NAME_NORMALIZED_SPOT_MEAN = f"Normalized.{COL_NAME_SPOT_MEAN}"
COL_NAME_NORMALIZED_BKG_MEDIAN = f"Normalized.{COL_NAME_BKG_MEDIAN}"
COL_NAME_NORMALIZED_SPOT_MEDIAN = f"Normalized.{COL_NAME_SPOT_MEDIAN}"
COL_NAME_NORMALIZED_BKG_STDDEV = f"Normalized.{COL_NAME_BKG_STDDEV}"
COL_NAME_NORMALIZED_SPOT_STDDEV = f"Normalized.{COL_NAME_SPOT_STDDEV}"
COL_NAME_NORMALIZED_BKG_SUM = f"Normalized.{COL_NAME_BKG_SUM}"
COL_NAME_NORMALIZED_SPOT_SUM = f"Normalized.{COL_NAME_SPOT_SUM}"
COLUMN_NORMALIZATION = {
COL_NAME_BKG_MEAN: COL_NAME_NORMALIZED_BKG_MEAN,
COL_NAME_SPOT_MEAN: COL_NAME_NORMALIZED_SPOT_MEAN,
COL_NAME_BKG_MEDIAN: COL_NAME_NORMALIZED_BKG_MEDIAN,
COL_NAME_SPOT_MEDIAN: COL_NAME_NORMALIZED_SPOT_MEDIAN,
COL_NAME_BKG_STDDEV: COL_NAME_NORMALIZED_BKG_STDDEV,
COL_NAME_SPOT_STDDEV: COL_NAME_NORMALIZED_SPOT_STDDEV,
COL_NAME_BKG_SUM: COL_NAME_NORMALIZED_BKG_SUM,
COL_NAME_SPOT_SUM: COL_NAME_NORMALIZED_SPOT_SUM,
}