Parsing the numerical output from Sensovation SensoSpot image analysis.
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from collections import namedtuple
import pytest
ExposureSetting = namedtuple("ExposureSetting", ["channel", "time"])
def test_split_data_frame(data_frame_with_params):
from sensospot_data.utils import split_data_frame
result = split_data_frame(data_frame_with_params, "Well.Row")
assert set(result.keys()) == set("ABC")
for key, value_df in result.items():
assert set(value_df["Well.Row"].unique()) == {key}
def test_infer_exposure_from_parameters(data_frame_with_params):
from sensospot_data.utils import _set_exposure_data_from_parameters
result = _set_exposure_data_from_parameters(data_frame_with_params)
assert all(result["Exposure.Channel"] == result["Parameters.Channel"])
assert all(result["Exposure.Time"] == result["Parameters.Time"])
def test_infer_exposure_from_parameters_raises_error(
data_frame_without_params,
):
from sensospot_data.utils import _set_exposure_data_from_parameters
with pytest.raises(ValueError) as excinfo:
_set_exposure_data_from_parameters(data_frame_without_params)
assert str(excinfo.value).startswith("Exposure Map: measurement")
def test_apply_exposure_map(data_frame_with_params):
from sensospot_data.utils import apply_exposure_map
exposure_map = {
1: ExposureSetting("Cy3", 100),
2: ExposureSetting("Cy5", 15),
3: ExposureSetting("Cy5", 150),
}
result = apply_exposure_map(data_frame_with_params, exposure_map)
for key, value in exposure_map.items():
mask = result["Exposure.Id"] == key
partial = result.loc[mask]
assert set(partial["Exposure.Channel"].unique()) == {value.channel}
assert set(partial["Exposure.Time"].unique()) == {value.time}
def test_apply_exposure_map_raises_error(data_frame_with_params):
from sensospot_data.utils import apply_exposure_map
exposure_map = {
1: ExposureSetting("Cy3", 100),
2: ExposureSetting("Cy5", 15),
"X": ExposureSetting("Cy5", 150),
}
with pytest.raises(ValueError) as excinfo:
apply_exposure_map(data_frame_with_params, exposure_map)
assert str(excinfo.value).startswith("Exposure Map differs")
def test_apply_exposure_map_from_parameters(data_frame_with_params):
from sensospot_data.utils import apply_exposure_map
result = apply_exposure_map(data_frame_with_params, None)
assert all(result["Exposure.Channel"] == result["Parameters.Channel"])
assert all(result["Exposure.Time"] == result["Parameters.Time"])
def test_apply_exposure_map_from_parameters_raises_error(
data_frame_without_params,
):
from sensospot_data.utils import apply_exposure_map
with pytest.raises(ValueError) as excinfo:
apply_exposure_map(data_frame_without_params, None)
assert str(excinfo.value).startswith("Exposure Map: measurement")