Sensospot Tools

Some small tools for working with parsed Sensospot data.

Selecting and spliting a pandas data frame

select(data: DataFrame, column: str, value: Any) -> DataFrame

Selects rows of a dataframe based on a value in a column

Example:


    from sensospot_tools import select

    print(data)
        category  value
    0      dog      1
    1      cat      2
    2    horse      3
    3      cat      4

    print(select(data, "category", "cat"))
          category  value
        1      cat      2
        3      cat      4

split(data: DataFrame, column: str) -> Iterator[tuple[Any, DataFrame]]

Splits a data frame on unique values in a column

Returns an iterator where each result is key-value-pair. The key is the unique value used for the split, the value is a slice of the dataframe selected by the unique value contained in the column.

Example:


    from sensospot_tools import split

    print(data)
        category  value
    0      dog      1
    1      cat      2
    2    horse      3
    3      cat      4

    result = dict( split(data, column="category") )

    print(result["dog"])
        category  value
    0      dog      1

    print(result["cat"])
        category  value
    1      cat      2
    3      cat      4

    print(result["horse"])
        category  value
    2    horse      3

Working with data with multiple exposure times

select_hdr_data(data: DataFrame, spot_id_columns: list[str], time_column: str, overflow_column: str) -> DataFrame:

Selects the data for increased dynamic measurement range.

To increase the dynamic range of a measurement, multiple exposures of one microarray might be taken.

This function selects the data of only one exposure time per spot, based on the information if the spot is in overflow. It starts with the weakest signals (longest exposure time) first and chooses the next lower exposure time, if the result in the overflow_column is True.

This is done for each spot, and therfore a spot needs a way to be identified across multiple exposure times. Examples for this are: - for a single array: the spot id (e.g. "Pos.Id") - for multiple arrays: the array position and the spot id (e.g. "Well.Name" and "Pos.Id") - for multiple runs: the name of the run, array position and the spot id (e.g. "File.Name", "Well.Name" and "Pos.Id")

The function will raise a KeyError if any of the provided column names is not present in the data frame

normalize(data: DataFrame, normalized_time: Union[int, float], time_column: str, value_columns: list[str], template: str) -> DataFrame:

normalizes values to a normalized exposure time

Will raise a KeyError, if any column is not in the data frame; raises ValueError if no template string was provided.

Development

To install the development version of Sensospot Tools:

git clone https://git.cpi.imtek.uni-freiburg.de/holgi/sensospot_tools.git

# create a virtual environment and install all required dev dependencies
cd sensospot_tools
make devenv

To run the tests, use make tests or make coverage for a complete report.

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