Holger Frey
2 years ago
6 changed files with 231 additions and 21 deletions
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<?xml version="1.0" encoding="utf-8"?> |
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<?xml-stylesheet type='text/xsl' href='220307_SN0801_CHECK-01_SL1,11,9,14_MS_1_1.xsl'?> |
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<ScanJobResult ID="220307_SN0801_CHECK-01_SL1,11,9,14_MS_1_1" Rack="Calibration Slide - 4 Slides"> |
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<StartTime>3/7/2022 5:31:40 PM</StartTime> |
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<FinishTime>3/7/2022 5:33:45 PM</FinishTime> |
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<ExperimentResults> |
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<AssayResult ID="A01" Type="Calibration Check_v1.2.4.3c"> |
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<ReferencePatternResult Status="Valid" Rotation="0.23807670284076057" Scaling="0.998411582643245" PositionTopLeft.X="230.07833862304688" PositionTopLeft.Y="232.07130432128906" /> |
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<RelationArrayToRefPattern OffsetMm.X="0.018938772094637457" OffsetMm.Y="-0.0071761016171074665" ScalingFactor="1.001991960802898" RotationAngleDeg="0" /> |
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<ChannelConfig1> |
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<Status ErrorID="0" Message="no error" /> |
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<ChannelDescription>Cy3/Cy5 Green</ChannelDescription> |
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<Timestamp>3/7/2022 5:31:47 PM</Timestamp> |
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<ImageFileName>220307_SN0801_CHECK-01_SL1,11,9,14_MS_1_1_A01_1.tif</ImageFileName> |
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<ImageResolutionPixPerMm>150.3716670323235</ImageResolutionPixPerMm> |
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<SystemImageResolutionPixPerMm>150.6109</SystemImageResolutionPixPerMm> |
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<Transform> |
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<M11>150.66990076589988</M11> |
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<M12>-0.62607054037768517</M12> |
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<M21>0.62607054037768517</M21> |
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<M22>150.66990076589988</M22> |
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<Dx>227.22602466270919</Dx> |
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<Dy>233.1385439330713</Dy> |
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</Transform> |
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<SpotResults> |
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<Spot ID="1"> |
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<Result Label="Pos.X" Type="System.Int32" Value="228" /> |
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<Result Label="Pos.Y" Type="System.Int32" Value="232" /> |
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<Result Label="Bkg.Mean" Type="System.Double" Value="0.23538493173349712" /> |
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<?xml version="1.0" encoding="utf-8"?> |
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<!--Algorithm configuration--> |
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<Algorithm> |
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<BrightnessCheck> |
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<Settings Active="False" MinimumBrightnessPercent="70" MaximumBrightnessPercent="100" HistogrammPercent="95" OverrideMaximumPixelValue="0" SubSampleX="2" SubSampleY="2" /> |
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</BrightnessCheck> |
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<Shading> |
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<Settings Active="true" /> |
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</Shading> |
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<OrientationDetection> |
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<Settings Active="True" HoughCircleSensitivitySliderUsed="True" BlobMethod="Classic,HoughCircle" HoughCircleGamma="1.1" HoughCircleScale="1" HoughCircleMinDist="5" HoughCircleCannyThresh="100" HoughCircleAccumThresh="15" HoughCircleMinRadius="5" HoughCircleMaxRadius="30" HoughCircleSmoothRadius="0" HoughCircleDilationRadius="3" HoughCircleSensitivitySlider="2" RefPatternFittingMethod="ICP" AdvancedMaxMissingSpots="1" BinThresholdPercent="40" JoinRadius="2" OpeningNeighborhoodSizeXY="7" BlobAreaMin="50"> |
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</Settings> |
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<DebugSettings ImageDebugAODResult="0" ImageDebugAODBinary="0" ImageDebugAODMorphed="1" ShowResults="1" ShowSearchOrder="1" ShowDistances="1" /> |
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</OrientationDetection> |
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<Flip> |
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<Settings Direction="FLIP_NONE" /> |
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</Flip> |
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<MicroArraySpotFinding> |
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<Settings Active="True" BkgAvgAreaPix="10" HistogramPerCent="10" BinarizationThresholdOffsetPerc="7" SpotImageSubSampling="1" ApertureWidth="3" MinArea="50" MinSpotDiameterMm="0.15" SpotFindingSensitivity="10" SpotShape="CIRCLE" SelectionPreference="Compactest" InsideGridRectCriteria="Rectangle" BlobbingActive="True" CircleDetectionActive="True" MeanBeforeEdgeDetectionActive="False" CannyThreshold="50" MinMatchQuality="0.35" CircleMatchExponent="1" /> |
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</MicroArraySpotFinding> |
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<MicroArrayAnalysis> |
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<Settings Active="True" MinBkgThresholdPerc="1" MaxSpotThresholdPerc="100" AutoAdjustAnalysisDiameter="True" MinSpotSizeMm="0.18" MaxSpotSizeMm="0.28" AutoAdjustTolerancePercentage="10"> |
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</Settings> |
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</MicroArrayAnalysis> |
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<Registration> |
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<Settings XmaxDisplacementMm="0.2" YmaxDisplacementMm="0.2" MinWithForStitchingMm="0.5" MinHeightForStitchingMm="0.5" XcutterAreaSizeMm="0.4" YcutterAreaSizeMm="0.4" RegistrationTypeFlair="TMWithSobelAndDustFilter" RegistrationTypeClair="TMWeightEdges" ROIMarginHeight="0.5" ROIMarginWidth="0.5"> |
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</Settings> |
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</Registration> |
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</Algorithm> |
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<?xml version="1.0" encoding="utf-8"?> |
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<!--Definition of the microarray and the--> |
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<!--embedded reference pattern--> |
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<MicroArray> |
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<Layout NofSpotsX="10" NofSpotsY="10" SpotDistMmX="0.353" SpotDistMmY="0.349" /> |
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<ReferencePattern ReferencePointOffsetMm.X="0.689155651736256" ReferencePointOffsetMm.Y="1.5170187539612119"> |
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<Features MarkerType="BRIGHT"> |
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</Features> |
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<Tolerances ScalePercent="5" RotationPercent="5"> |
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<CIRCLE PositionMm="0.06" SizePercent="50" SimpleCompactnessMax="17" EccentricityMax="0.2" /> |
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<RECT PositionMm="0.1" SizePercent="30" SimpleCompactnessMin="13" SimpleCompactnessMax="22" EccentricityMin="0.25" EccentricityMax="0.4"> |
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</RECT> |
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</Tolerances> |
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<Shapes> |
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<Shape0 ShapeType="CIRCLE" PosMm.X="0" PosMm.Y="0" DiameterMm="0.203"> |
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</Shape0> |
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<Shape1 ShapeType="CIRCLE" PosMm.X="3.161" PosMm.Y="3.124" DiameterMm="0.21"> |
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</Shape1> |
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<Shape2 ShapeType="CIRCLE" PosMm.X="0.019" PosMm.Y="3.153" DiameterMm="0.203"> |
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</Shape2> |
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<Shape3 ShapeType="CIRCLE" PosMm.X="3.129" PosMm.Y="-0.016" DiameterMm="0.21"> |
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</Shape3> |
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<Shape4 ShapeType="CIRCLE" PosMm.X="0.013" PosMm.Y="2.794" DiameterMm="0.21"> |
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</Shape4> |
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</Shapes> |
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</ReferencePattern> |
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<RelationArrayToRefPattern OffsetMm.X="0.0053912827593903812" OffsetMm.Y="-0.00040235694948398404" ScalingFactor="1.001991960802898" RotationAngleDeg="0"> |
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</RelationArrayToRefPattern> |
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<SpotsLayout> |
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<Spot Number="1" Id="" Name="" /> |
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<Spot Number="2" Id="" Name="" /> |
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<Spot Number="3" Id="" Name="" /> |
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<Spot Number="4" Id="" Name="" /> |
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<Spot Number="5" Id="" Name="" /> |
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<Spot Number="6" Id="" Name="" /> |
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<Spot Number="7" Id="" Name="" /> |
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<Spot Number="8" Id="" Name="" /> |
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<Spot Number="9" Id="" Name="" /> |
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<Spot Number="10" Id="" Name="" /> |
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<Spot Number="11" Id="" Name="" /> |
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<Spot Number="12" Id="" Name="" /> |
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<Spot Number="13" Id="" Name="" /> |
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<Spot Number="14" Id="" Name="" /> |
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<Spot Number="15" Id="" Name="" /> |
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<Spot Number="16" Id="" Name="" /> |
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<Spot Number="17" Id="" Name="" /> |
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<Spot Number="18" Id="" Name="" /> |
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<Spot Number="19" Id="" Name="" /> |
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<Spot Number="20" Id="" Name="" /> |
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<Spot Number="21" Id="" Name="" /> |
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<Spot Number="22" Id="" Name="" /> |
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<Spot Number="23" Id="" Name="" /> |
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<Spot Number="24" Id="" Name="" /> |
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<Spot Number="25" Id="" Name="" /> |
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<Spot Number="26" Id="" Name="" /> |
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<Spot Number="27" Id="" Name="" /> |
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<Spot Number="28" Id="" Name="" /> |
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<Spot Number="29" Id="" Name="" /> |
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<Spot Number="30" Id="" Name="" /> |
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<Spot Number="31" Id="" Name="" /> |
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<Spot Number="32" Id="" Name="" /> |
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<Spot Number="33" Id="" Name="" /> |
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<Spot Number="34" Id="" Name="" /> |
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<Spot Number="35" Id="" Name="" /> |
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<Spot Number="36" Id="" Name="" /> |
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<Spot Number="37" Id="" Name="" /> |
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<Spot Number="38" Id="" Name="" /> |
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<Spot Number="39" Id="" Name="" /> |
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<Spot Number="40" Id="" Name="" /> |
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<Spot Number="41" Id="" Name="" /> |
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<Spot Number="42" Id="" Name="" /> |
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<Spot Number="43" Id="" Name="" /> |
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<Spot Number="44" Id="" Name="" /> |
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<Spot Number="45" Id="" Name="" /> |
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<Spot Number="46" Id="" Name="" /> |
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<Spot Number="47" Id="" Name="" /> |
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<Spot Number="48" Id="" Name="" /> |
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<Spot Number="49" Id="" Name="" /> |
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<Spot Number="50" Id="" Name="" /> |
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<Spot Number="51" Id="" Name="" /> |
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<Spot Number="52" Id="" Name="" /> |
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<Spot Number="53" Id="" Name="" /> |
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<Spot Number="54" Id="" Name="" /> |
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<Spot Number="55" Id="" Name="" /> |
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<Spot Number="56" Id="" Name="" /> |
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<Spot Number="57" Id="" Name="" /> |
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<Spot Number="58" Id="" Name="" /> |
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<Spot Number="59" Id="" Name="" /> |
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<Spot Number="60" Id="" Name="" /> |
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<Spot Number="61" Id="" Name="" /> |
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<Spot Number="62" Id="" Name="" /> |
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<Spot Number="63" Id="" Name="" /> |
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<Spot Number="64" Id="" Name="" /> |
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<Spot Number="65" Id="" Name="" /> |
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<Spot Number="66" Id="" Name="" /> |
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<Spot Number="67" Id="" Name="" /> |
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<Spot Number="68" Id="" Name="" /> |
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<Spot Number="69" Id="" Name="" /> |
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<Spot Number="70" Id="" Name="" /> |
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<Spot Number="71" Id="" Name="" /> |
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<Spot Number="72" Id="" Name="" /> |
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<Spot Number="73" Id="" Name="" /> |
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<Spot Number="74" Id="" Name="" /> |
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<Spot Number="75" Id="" Name="" /> |
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<Spot Number="76" Id="" Name="" /> |
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<Spot Number="77" Id="" Name="" /> |
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<Spot Number="78" Id="" Name="" /> |
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<Spot Number="79" Id="" Name="" /> |
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<Spot Number="80" Id="" Name="" /> |
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<Spot Number="81" Id="" Name="" /> |
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<Spot Number="82" Id="" Name="" /> |
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<Spot Number="83" Id="" Name="" /> |
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<Spot Number="84" Id="" Name="" /> |
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<Spot Number="85" Id="" Name="" /> |
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<Spot Number="86" Id="" Name="" /> |
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<Spot Number="87" Id="" Name="" /> |
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<Spot Number="88" Id="" Name="" /> |
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<Spot Number="89" Id="" Name="" /> |
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<Spot Number="90" Id="" Name="" /> |
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<Spot Number="91" Id="" Name="" /> |
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<Spot Number="92" Id="" Name="" /> |
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<Spot Number="93" Id="" Name="" /> |
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<Spot Number="94" Id="" Name="" /> |
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<Spot Number="95" Id="" Name="" /> |
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<Spot Number="96" Id="" Name="" /> |
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<Spot Number="97" Id="" Name="" /> |
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<Spot Number="98" Id="" Name="" /> |
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<Spot Number="99" Id="" Name="" /> |
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<Spot Number="100" Id="" Name="" /> |
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</SpotsLayout> |
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</MicroArray> |
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|
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<?xml version="1.0"?> |
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<Assay> |
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<Channels> |
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<ChannelConfig1 IlluminationID="4" Description="Cy3/Cy5 Green" ExposureTimeMs="100" Intensity="100"> |
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</ChannelConfig1> |
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<ChannelConfig2 IlluminationID="4" Description="Cy3/Cy5 Green" ExposureTimeMs="10" Intensity="100"> |
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</ChannelConfig2> |
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<ChannelConfig3 IlluminationID="3" Description="Cy3/Cy5 Red" ExposureTimeMs="60" Intensity="100"> |
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</ChannelConfig3> |
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<ChannelConfig4 IlluminationID="3" Description="Cy3/Cy5 Red" ExposureTimeMs="6" Intensity="100"> |
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</ChannelConfig4> |
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</Channels> |
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<Components MicroArray="Calibration Check 4e" AlgoConfig="Calibration Check 4e"> |
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</Components> |
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<DebugSwitches DoImageProcessing="True" DoDarkImageCorrection="False" DoFullRangeAdaption="True" RestoreSaturation="True" SaveResultAsCSVFile="True" SaveResultAsXmlFile="True" DoShadingCorrection="True" DoRemap="True"> |
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</DebugSwitches> |
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<RefPattern ChannelConfig="Channel1"> |
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</RefPattern> |
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<WorkbookAnalysis Active="True" UseSingleWorkbook="YES" TemplateWorkbookName="2022-03-15 Calibration Check v1.2.5.4e.xlsx" PasteWorksheetName="Input_Data" PasteStartingCell="AA300" ParameterWorksheetName="parameter" ResultWorksheetName="Workflow" KeepDataWorkbookOpen="NO"> |
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</WorkbookAnalysis> |
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</Assay> |
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|
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<?xml version="1.0"?> |
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<Rack Version="2"> |
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<Reference RefPosXMm="103.5" RefPosYMm="67.5" RefPosZMm="3.8" XAxisInverted="True" YAxisInverted="True" ScanAreaValidationActive="True"> |
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</Reference> |
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<ChannelSettings> |
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<ChannelSetting1 ExposureTimeMs="30" IlluminationId="3" Intensity="100"> |
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</ChannelSetting1> |
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<ChannelSetting2 ExposureTimeMs="30" IlluminationId="4" Intensity="100"> |
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</ChannelSetting2> |
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</ChannelSettings> |
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<PlateHolder HolderType="SinglePlateHolder"> |
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</PlateHolder> |
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<Layout OffsetXMm="17" OffsetYMm="19" OffsetZMm="5.84" NofWellsX="1" NofWellsY="4" WellDistXMm="9" WellDistYMm="9" WellDiameterMm="5" WellSizeXMm="1" WellSizeYMm="1" WellType="Circle"> |
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</Layout> |
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<Flip> |
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<Settings Direction="FLIP_NONE"> |
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</Settings> |
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</Flip> |
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<Sharpness XBorderPercent="0.333333343" YBorderPercent="0.333333343"> |
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</Sharpness> |
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</Rack> |
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<?xml version="1.0"?> |
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<Rack Version="2"> |
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<Reference RefPosXMm="103.5" RefPosYMm="67.5" RefPosZMm="3.8" XAxisInverted="True" YAxisInverted="True"> |
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</Reference> |
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<ChannelSettings> |
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<ChannelSetting1 ExposureTimeMs="100" IlluminationId="3" Intensity="100"> |
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</ChannelSetting1> |
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<ChannelSetting2 ExposureTimeMs="50" IlluminationId="4" Intensity="100"> |
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</ChannelSetting2> |
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</ChannelSettings> |
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<PlateHolder HolderType="SinglePlateHolder"> |
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</PlateHolder> |
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<Layout OffsetXMm="12.5" OffsetYMm="10" OffsetZMm="3.42" NofWellsX="12" NofWellsY="8" WellDistXMm="9" WellDistYMm="9" WellDiameterMm="5" WellSizeXMm="1" WellSizeYMm="1" WellType="Circle"> |
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</Layout> |
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<DigitalPlate Create="False" BkgndColor="Black"> |
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</DigitalPlate> |
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<Flip> |
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<Settings Direction="FLIP_NONE"> |
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</Settings> |
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</Flip> |
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</Rack> |
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