Holger Frey
2 years ago
8 changed files with 47 additions and 106 deletions
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<?xml version="1.0" encoding="utf-8"?> |
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<!--Algorithm configuration--> |
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<Algorithm> |
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<BrightnessCheck> |
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<Settings Active="False" MinimumBrightnessPercent="70" MaximumBrightnessPercent="100" HistogrammPercent="95" OverrideMaximumPixelValue="0" SubSampleX="2" SubSampleY="2" /> |
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</BrightnessCheck> |
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<Shading> |
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<Settings Active="true" /> |
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</Shading> |
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<OrientationDetection> |
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<Settings Active="True" HoughCircleSensitivitySliderUsed="True" BlobMethod="Classic,HoughCircle" HoughCircleGamma="1.1" HoughCircleScale="1" HoughCircleMinDist="5" HoughCircleCannyThresh="100" HoughCircleAccumThresh="15" HoughCircleMinRadius="5" HoughCircleMaxRadius="30" HoughCircleSmoothRadius="0" HoughCircleDilationRadius="3" HoughCircleSensitivitySlider="2" RefPatternFittingMethod="ICP" BinThresholdPercent="2" JoinRadius="2" OpeningNeighborhoodSizeXY="7" BlobAreaMin="50"> |
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</Settings> |
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<DebugSettings ImageDebugAODResult="0" ImageDebugAODBinary="0" ImageDebugAODMorphed="1" ShowResults="1" ShowSearchOrder="1" ShowDistances="1" /> |
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</OrientationDetection> |
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<Flip> |
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<Settings Direction="FLIP_NONE" /> |
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</Flip> |
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<MicroArraySpotFinding> |
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<Settings Active="True" BkgAvgAreaPix="10" HistogramPerCent="10" BinarizationThresholdOffsetPerc="7" SpotImageSubSampling="1" ApertureWidth="3" MinArea="50" MinSpotDiameterMm="0.2" SpotFindingSensitivity="10" SpotShape="CIRCLE" SelectionPreference="COMPACTEST" InsideGridRectCriteria="RECTANGLE" BlobbingActive="True" CircleDetectionActive="True" MeanBeforeEdgeDetectionActive="False" CannyThreshold="50" MinMatchQuality="0.4" CircleMatchExponent="1" /> |
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</MicroArraySpotFinding> |
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<MicroArrayAnalysis> |
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<Settings Active="True" MinBkgThresholdPerc="1" MaxSpotThresholdPerc="100" AutoAdjustAnalysisDiameter="False" MinSpotSizeMm="0.2" MaxSpotSizeMm="0.28" AutoAdjustTolerancePercentage="10"> |
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</Settings> |
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</MicroArrayAnalysis> |
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</Algorithm> |
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<?xml version="1.0" encoding="utf-8"?> |
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<!--Definition of the microarray and the--> |
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<!--embedded reference pattern--> |
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<MicroArray> |
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<Layout NofSpotsX="10" NofSpotsY="10" SpotDistMmX="0.303" SpotDistMmY="0.297" /> |
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<ReferencePattern ReferencePointOffsetMm.X="1.6392982179205673" ReferencePointOffsetMm.Y="2.0253288169139632"> |
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<Features MarkerType="BRIGHT"> |
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</Features> |
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<Tolerances ScalePercent="5" RotationPercent="5"> |
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<CIRCLE PositionMm="0.06" SizePercent="50" SimpleCompactnessMax="17" EccentricityMax="0.2" /> |
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<RECT PositionMm="0.1" SizePercent="30" SimpleCompactnessMin="13" SimpleCompactnessMax="22" EccentricityMin="0.25" EccentricityMax="0.4"> |
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</RECT> |
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</Tolerances> |
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<Shapes> |
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<Shape0 ShapeType="CIRCLE" PosMm.X="0" PosMm.Y="0" DiameterMm="0.187"> |
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</Shape0> |
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<Shape1 ShapeType="CIRCLE" PosMm.X="2.728" PosMm.Y="2.682" DiameterMm="0.184"> |
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</Shape1> |
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<Shape2 ShapeType="CIRCLE" PosMm.X="2.701" PosMm.Y="-0.021" DiameterMm="0.187"> |
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</Shape2> |
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<Shape3 ShapeType="CIRCLE" PosMm.X="0.024" PosMm.Y="2.7" DiameterMm="0.191"> |
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</Shape3> |
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</Shapes> |
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</ReferencePattern> |
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<RelationArrayToRefPattern OffsetMm.X="0" OffsetMm.Y="0" ScalingFactor="0.99430589150056758" RotationAngleDeg="-0.13126175132547502"> |
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</RelationArrayToRefPattern> |
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</MicroArray> |
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@@ -1,19 +0,0 @@
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<?xml version="1.0"?> |
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<Assay> |
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<Channels> |
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<ChannelConfig1 IlluminationID="4" Description="Cy3/Cy5 Green" ExposureTimeMs="100" Intensity="100"> |
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</ChannelConfig1> |
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<ChannelConfig2 IlluminationID="3" Description="Cy3/Cy5 Red" ExposureTimeMs="150" Intensity="100"> |
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</ChannelConfig2> |
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<ChannelConfig3 IlluminationID="3" Description="Cy3/Cy5 Red" ExposureTimeMs="15" Intensity="100"> |
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</ChannelConfig3> |
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</Channels> |
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<Components MicroArray="S QC 10x10 Cy3 100ms Cy5 150-15ms" AlgoConfig="S QC 10x10 Cy3 100ms Cy5 150-15ms"> |
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</Components> |
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<DebugSwitches DoImageProcessing="True" DoDarkImageCorrection="True" SaveResultAsCSVFile="True" SaveResultAsXmlFile="True"> |
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</DebugSwitches> |
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<RefPattern ChannelConfig="Channel1"> |
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</RefPattern> |
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<WorkbookAnalysis Active="True" UseSingleWorkbook="YES" TemplateWorkbookName="160212_wb10x10_Spectra_V3_Ver03.2.3.xlsx" PasteWorksheetName="Input_Data" PasteStartingCell="W300" ParameterWorksheetName="parameter" ResultWorksheetName="Net Intensity" KeepDataWorkbookOpen="NO"> |
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</WorkbookAnalysis> |
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</Assay> |
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